Zorbax StableBond

Zorbax StableBond Guard Columns from Agilent Technologies

Choosing to use guard columns can help reduce operating expenses, by reducing the frequency of analytical column replacement. The guard column prevents damage caused by particulate matter and strongly adsorbed material. To maintain an adequate capacity for sample impurities, choose a guard column with an internal diameter similar to the column internal diameter.

Ideally, the packing of the guard column should be the same as the analytical column so that the chromatography of the analytical column is not altered. Guard columns contribute to the separation, so you should include a guard column inline during method development. Agilent UHPLC guards provide protection for high-efficiency Poroshell 120and ZORBAX RRHD and RRHT columns, without reducing performance.

ZORBAX 80Å StableBond columns are made using bulky, patented, unique silanes that sterically protect the siloxane bond. Acid labile endcapping reagents are not used. The result is vastly improved column life and extraordinary chemical and temperature stability in the pH 1–6 range for a wide variety of phases (SB-Aq, SB-C3, SB-CN, SB-Phenyl, SB-C8, and SB-C18). Excellent peak shape is expected for many basic compounds because of the ultrapure Rx-SIL support.